Involvement of recF , recO , and recR Genes in UV-Radiation Mutagenesis of Escherichia coli
نویسندگان
چکیده
منابع مشابه
Serratia Exposed to UV Radiation Show Increase In Survival Capabilities
The recF, recO, and recR genes were originally identified as those affecting the RecF pathway ofrecombination in Escherichia coli cells. Several lines of evidence suggest that the recF, recO, andrecR genes function at the same step of recombination and postreplication repair. In this work,we report that null mutations in recF, recO, or recR greatly reduce UV-radiation mutagenesis<lb...
متن کاملPhage X Has an Analog of Escherichia coli rec 0 , recR and recF Genes
The RecF pathway catalyzes generalized recombination in Escherichia coli that is mutant for recBC, sbcB and sbcC. This pathway operating on conjugational recombination requires the recA, recF, recJ, recN, rec0, recQ, recR, ruvA, ruvB and ruvC genes. In contrast, X mutant for its own recombination genes, int, reda and red& requires only the recA and reg genes to recombine efficiently in recBC sb...
متن کاملRecO acts with RecF and RecR to protect and maintain replication forks blocked by UV-induced DNA damage in Escherichia coli.
In Escherichia coli, recF and recR are required to stabilize and maintain replication forks arrested by UV-induced DNA damage. In the absence of RecF, replication fails to recover, and the nascent lagging strand of the arrested replication fork is extensively degraded by the RecQ helicase and RecJ nuclease. recO mutants are epistatic with recF and recR with respect to recombination and survival...
متن کاملRoles of RecJ, RecO, and RecR in RecET-mediated illegitimate recombination in Escherichia coli.
We analyzed effects of overexpression of RecE and RecT on illegitimate recombination during prophage induction in Escherichia coli and found that frequencies of spontaneous and UV-induced illegitimate recombination are enhanced by coexpression of RecE and RecT in the wild type, but the enhanced recombination was reduced by recJ, recO, or recR mutation. The results indicated that RecET-mediated ...
متن کاملrecF and recR are required for the resumption of replication at DNA replication forks in Escherichia coli.
Escherichia coli containing a mutation in recF are hypersensitive to UV. However, they exhibit normal levels of conjugational or transductional recombination unless the major pathway (recBC) is defective. This implies that the UV sensitivity of recF mutants is not due to a defect in recombination such as occurs during conjugation or transduction. Here, we show that when replication is disrupted...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1998
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.180.7.1766-1770.1998